Journal article
Micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer
PL Mok, SN Leow, AEH Koh, HH Mohd Nizam, SLS Ding, C Luu, R Ruhaslizan, HS Wong, WHWA Halim, MH Ng, RBH Idrus, SR Chowdhury, CML Bastion, SK Subbiah, A Higuchi, AA Alarfaj, KY Then
International Journal of Molecular Sciences | MDPI | Published : 2017
DOI: 10.3390/ijms18020345
Abstract
Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of th..
View full abstractGrants
Awarded by Ministry of Higher Education, Malaysia
Funding Acknowledgements
This research was supported by the Universiti Kebangsaan Malaysia-Cryocord Sdn. Bhd. Research Incentive Grant (Industri-2011-048), Ministry of Science, Technology and Innovation (MOSTI), Malaysia through the Science Fund (Grant No.: 5450817), Fundamental Research Grants Scheme (FRGS), Ministry of Education, Malaysia (Grant No.: 5524401), and the Putra Grants of Universiti Putra Malaysia, Malaysia (Grant No.: 9436300 and 9503900). The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for funding this research group (Project No.: RG-1435-065).