Journal article
Characterisation of microbial communities within aggressive prostate cancer tissues
MA Yow, SN Tabrizi, G Severi, DM Bolton, J Pedersen, GG Giles, MC Southey
Infectious Agents and Cancer | BMC | Published : 2017
Abstract
Background: An infectious aetiology for prostate cancer has been conjectured for decades but the evidence gained from questionnaire-based and sero-epidemiological studies is weak and inconsistent, and a causal association with any infectious agent is not established. We describe and evaluate the application of new technology to detect bacterial and viral agents in high-grade prostate cancer tissues. The potential of targeted 16S rRNA gene sequencing and total RNA sequencing was evaluated in terms of its utility to characterise microbial communities within high-grade prostate tumours. Methods: Two different Massively Parallel Sequencing (MPS) approaches were applied. First, to capture and enr..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council (APP504702), the Prostate Cancer Foundation of Australia (projects YIG19 and PG2709) and the Austin Urology Research Foundation supported by the Urologists of the Austin Hospital, Melbourne, Victoria, Australia. The authors would like to express their appreciation to the study participants who kindly donated tissue to the Australian Prostate Cancer BioResource. The Australian Prostate Cancer BioResource is supported by the National Health and Medical Research Council of Australia Enabling Grant (no. 614296) and by a grant from the Prostate Cancer Foundation Australia.