Long-Distance Axonal Growth and Protracted Functional Maturation of Neurons Derived from Human Induced Pluripotent Stem Cells After Intracerebral Transplantation
Jonathan C Niclis, Christopher Turner, Jennifer Durnall, Stuart McDougal, Jessica A Kauhausen, Bryan Leaw, Mirella Dottori, Clare L Parish, Lachlan H Thompson
Stem Cells Translational Medicine | WILEY | Published : 2017
The capacity for induced pluripotent stem (iPS) cells to be differentiated into a wide range of neural cell types makes them an attractive donor source for autologous neural transplantation therapies aimed at brain repair. Translation to the in vivo setting has been difficult, however, with mixed results in a wide variety of preclinical models of brain injury and limited information on the basic in vivo properties of neural grafts generated from human iPS cells. Here we have generated a human iPS cell line constitutively expressing green fluorescent protein as a basis to identify and characterize grafts resulting from transplantation of neural progenitors into the adult rat brain. The result..View full abstract
Awarded by NHMRC
We thank Mong Tien and Haoyao Guo for expert technical assistance. C.P. is a Viertel Senior Research Fellow. This work was supported NHMRC project Grant 1042584. The Florey Institute of Neuroscience and Mental Health acknowledges the strong support from the Victorian Government and in particular the funding from the Operational Infrastructure Support Grant.