Effect of a novel quaternary ammonium silane on dentin protease activities

Abstract

Objectives Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. Methods Dentin beams were demineralized with 10% H3PO4 for 24 h. After baseline dry mass measurements, the beams were divided into 5 groups (N = 10) according to protease inhibitors. The beams were pre-treated for 2 min with 2% chlorhexidine (CHX), 2%, 5% or 10% QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calci..