Gene Function Analysis in the Ubiquitous Human Commensal and Pathogen Malassezia Genus
Giuseppe Ianiri, Anna F Averette, Joanne M Kingsbury, Joseph Heitman, Alexander Idnurm
mBio | AMER SOC MICROBIOLOGY | Published : 2016
The genus Malassezia includes 14 species that are found on the skin of humans and animals and are associated with a number of diseases. Recent genome sequencing projects have defined the gene content of all 14 species; however, to date, genetic manipulation has not been possible for any species within this genus. Here, we develop and then optimize molecular tools for the transformation of Malassezia furfur and Malassezia sympodialis using Agrobacterium tumefaciens delivery of transfer DNA (T-DNA) molecules. These T-DNAs can insert randomly into the genome. In the case of M. furfur, targeted gene replacements were also achieved via homologous recombination, enabling deletion of the ADE2 gene ..View full abstract
Awarded by HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
This work, including the efforts of Joseph Heitman, was funded by HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (AI50113-13 and AI39115-19). This work, including the efforts of Alexander Idnurm, was funded by Australian Research Council (ARC) and by Procter and Gamble (P&G).