Journal article
Inhibitor of Apoptosis Protein-1 Regulates Tumor Necrosis Factor–Mediated Destruction of Intestinal Epithelial Cells
T Grabinger, KJ Bode, J Demgenski, C Seitz, ME Delgado, F Kostadinova, C Reinhold, N Etemadi, S Wilhelm, M Schweinlin, K Hänggi, J Knop, C Hauck, H Walles, J Silke, H Wajant, U Nachbur, W W. Wei-Lynn, T Brunner
Gastroenterology | W B SAUNDERS CO-ELSEVIER INC | Published : 2017
Abstract
Background and Aims Tumor necrosis factor (TNF) is a cytokine that promotes inflammation and contributes to pathogenesis of inflammatory bowel diseases. Unlike other cells and tissues, intestinal epithelial cells undergo rapid cell death upon exposure to TNF, by unclear mechanisms. We investigated the roles of inhibitor of apoptosis proteins (IAPs) in the regulation of TNF-induced cell death in the intestinal epithelium of mice and intestinal organoids. Methods RNA from cell lines and tissues was analyzed by quantitative polymerase chain reaction, protein levels were analyzed by immunoblot assays. BIRC2 (also called cIAP1) was expressed upon induction from lentiviral vectors in young adult m..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by research grants from the German Science Foundation (DFG) to Thomas Brunner (BR 3369/5-1, INST 38/500-1, INST 38/498-1), from National Health and Medical Research Council (NHMRC) to John Silke (433013, 541902), from the Deutsche Krebshilfe to Harald Wajant (111703), and the Swiss National Science Foundation to W. Wei-Lynn Wong (310030-138085). Thomas Grabinger, Carina Seitz, Konstantin J. Bode, and John Silke were supported by fellowships from the Research Training Group (RTG1331, DFG), InViTe and NHMRC (541901 and 1058190), respectively. Ueli Nachbur is supported by fellowships from the Swiss National Science Foundation (PA00P3_126249) and the Australian Research Council (FT130100166). This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC Independent Research Institutes Infrastructure Support Scheme (#361646).