Journal article
Rapid Chair-Side Test for Detection of Porphyromonas gingivalis
NM O'Brien-Simpson, K Burgess, JC Lenzo, GC Brammar, IB Darby, EC Reynolds
Journal of Dental Research | SAGE PUBLICATIONS INC | Published : 2017
Abstract
Porphyromonas gingivalis is a keystone pathogen of chronic periodontitis, and its intraoral levels have been shown to predict disease progression (activity). An accurate and sensitive chair-side (point of care) test to determine disease activity is critical for early intervention and clinical management of disease. This study aimed to develop a rapid, chair-side, saliva-based detection of P. gingivalis. Monoclonal antibodies (mAbs) to the A1-adhesin domain of the P. gingivalis RgpA-Kgp proteinase-adhesin complex were screened by enzyme-linked immunosorbent assay and microbial flow cytometry, with 2 mAbs shown to recognize all laboratory and clinical strains tested, without significantly cros..
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Funding Acknowledgements
This work was supported by the Department of Industry, Innovation and Science of the Australian Government. We thank CSL Ltd for preparation of the monoclonal antibodies and GC Corporation for production of the immunochromatographic detection device. We also thank Satoshi Tosaki for valuable discussion. The funders and companies that supplied material for the study had no role in study design, data handling, analysis, or manuscript preparation. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.