Journal article
A comparison of three quantitative methods to estimate G6PD activity in the chittagong hill tracts, Bangladesh
B Ley, MS Alam, JJ O'Donnell, MS Hossain, MG Kibria, N Jahan, WA Khan, K Thriemer, MD Chatfield, RN Price, JS Richards
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2017
Open access
Abstract
Background Glucose-6-phosphate-dehydrogenase-deficiency (G6PDd) is a major risk factor for primaquine- induced haemolysis. There is a need for improved point-of-care and laboratory-based G6PD diagnostics to unsure safe use of primaquine. Methods G6PD activities of participants in a cross-sectional survey in Bangladesh were assessed using two novel quantitative assays, the modified WST-8 test and the CareStart™ G6PD Biosensor (Access Bio), The results were compared with a gold standard UV spectrophotometry assay (Randox). The handheld CareStart™ Hb instrument (Access Bio) is designed to be a companion instrument to the CareStart™ G6PD biosensor, and its performance was compared to the well-va..
View full abstractGrants
Awarded by Wellcome Trust
Awarded by Bill & Melinda Gates Foundation
Funding Acknowledgements
The field and laboratory components of the study were supported by the Asia Pacific Malaria Elimination Network (APMEN), funded by the Australian Government Department of Foreign Affairs and Trade, and The Bill and Melinda Gates Foundation. RNP is funded by the Wellcome Trust (Senior Fellowship in Clinical Science, 091625), BL and KT by the Bill & Melinda Gates Foundation (OPRA-OPP1054404) and JSR by the National Health and Medical Research Council of Australia (Early Career Fellowship). The Burnet Institute is supported by the NHMRC Independent Research Institutes Infrastructure Support Scheme, and a Victoria State Government Operational Infrastructure Support grant. The International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b) is grateful to the Governments of Bangladesh, Canada, Sweden and the UK for providing core/unrestricted support. The CareStart (TM) G6PD Biosensors, CareStartTM Hb machines and corresponding strips were provided by Access Bio free of charge. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.