Journal article
Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem/stromal cells is restored by aldehyde dehydrogenase agonists
GD Kusuma, MH Abumaree, AV Perkins, SP Brennecke, B Kalionis
Scientific Reports | NATURE PORTFOLIO | Published : 2017
DOI: 10.1038/srep42397
Open access
Abstract
High resistance to oxidative stress is a common feature of mesenchymal stem/stromal cells (MSC) and is associated with higher cell survival and ability to respond to oxidative damage. Aldehyde dehydrogenase (ALDH) activity is a candidate "universal" marker for stem cells. ALDH expression was significantly lower in decidual MSC (DMSC) isolated from preeclamptic (PE) patients. ALDH gene knockdown by siRNA transfection was performed to create a cell culture model of the reduced ALDH expression detected in PE-DMSC. We showed that ALDH activity in DMSC is associated with resistance to hydrogen peroxide (H 2 O 2)-induced toxicity. Our data provide evidence that ALDH expression in DMSC is required ..
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Awarded by King Abdullah International Medical Research Centre
Funding Acknowledgements
We acknowledge the generosity of the mothers who consented to provide their placental tissues and the clinical research midwives at the Royal Women's Hospital, Sue Duggan and Moira Stewart for sample collection. We acknowledge Dr. Matthew Burton (Murdoch Children's Research Institute) for his expertise in flow cytometry troubleshooting and Dr. Sue Finch (Statistical Consulting Centre, The University of Melbourne) for her critical review of our statistical data analysis. We thank Janet Stevenson, Melissa Duggan, Anthony J. Borg, and Debora Singgih for their technical assistance. This work was supported by King Abdullah International Medical Research Centre (Grant No. RC08/114), the Royal Women's Hospital Foundation, and an Australian Stem Cell Centre postgraduate scholarship.