Single nucleotide polymorphism discrimination with and without an ethidium bromide intercalator
Renzo A Fenati, Ashley R Connolly, Amanda V Ellis
ANALYTICA CHIMICA ACTA | ELSEVIER | Published : 2017
Single nucleotide polymorphism (SNP) genotyping is an important aspect in understanding genetic variations. Here, we discriminate SNPs using toe-hold mediated displacement reactions. The biological target is an 80 nucleotide long double-stranded-DNA from the mtDNA HV1 region, associated with maternal ancestry. This target has been specially designed with a pendant toehold and a cationic fluorophore, ATTO 647N, as a reporter, produced in a polymerase chain reaction. Rates of reaction for the toehold-polymerase chain reaction products (TPPs) with their corresponding complementary displacing sequences, labelled with a Black Hole Quencher 1, followed the order TPP-Cytosine > TPP-Thymine > TPP-Ad..View full abstract
Related Projects (1)
Awarded by Australian Research Council
A. V. E is a recipient of a Future Fellowship Grant (No. FT130100211) from the Australian Research Council.