Journal article

Enzymatic Single-Chain Antibody Tagging A Universal Approach to Targeted Molecular Imaging and Cell Homing in Cardiovascular Disease

HT Ta, S Prabhu, E Leitner, F Jia, D von Elverfeldt, Katherine E Jackson, T Heidt, AKN Nair, H Pearce, C von zur Muhlen, X Wang, K Peter, CE Hagemeyer

Circulation Research | LIPPINCOTT WILLIAMS & WILKINS | Published : 2011

Abstract

RATIONALE: Antibody-targeted delivery of imaging agents can enhance the sensitivity and accuracy of current imaging techniques. Similarly, homing of effector cells to disease sites increases the efficacy of regenerative cell therapy while reducing the number of cells required. Currently, targeting can be achieved via chemical conjugation to specific antibodies, which typically results in the loss of antibody functionality and in severe cell damage. An ideal conjugation technique should ensure retention of antigen-binding activity and functionality of the targeted biological component. OBJECTIVE: To develop a biochemically robust, highly reproducible, and site-specific coupling method using t..

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Grants

Awarded by National Health and Medical Research Council (NHMRC)


Awarded by Department of Innovation, Industry, Science and Research; National Heart Foundation


Awarded by German Research Foundation


Awarded by National Heart Foundation


Awarded by Australian Research Council


Funding Acknowledgements

This work was funded by National Health and Medical Research Council (NHMRC) project and development grants 472665 and 472667; the International Science Linkages program (Grant CG130101) from the Department of Innovation, Industry, Science and Research; National Heart Foundation Grant-in-Aid G09M4345 (H.P., C.E.H.); the German Research Foundation (Mu 2727/3-1); National Heart Foundation Postdoctoral Fellowship PF 09M 4688 (H.T.T.); Australian Research Council Future Fellowship FT0992210 (K.P.); and NHMRC Career Development Award 472668 (C.E.H.). The study was supported in part by the Victorian Government's Operational Infrastructure Support Program.