Heterogeneous Differentiation Patterns of Individual CD8( ) T Cells
Carmen Gerlach, Jan C Rohr, Leila Perie, Nienke van Rooij, Jeroen WJ van Heijst, Arno Velds, Jos Urbanus, Shalin H Naik, Heinz Jacobs, Joost B Beltman, Rob J de Boer, Ton NM Schumacher
Science | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2013
Upon infection, antigen-specific CD8(+) T lymphocyte responses display a highly reproducible pattern of expansion and contraction that is thought to reflect a uniform behavior of individual cells. We tracked the progeny of individual mouse CD8(+) T cells by in vivo lineage tracing and demonstrated that, even for T cells bearing identical T cell receptors, both clonal expansion and differentiation patterns are heterogeneous. As a consequence, individual naïve T lymphocytes contributed differentially to short- and long-term protection, as revealed by participation of their progeny during primary versus recall infections. The discordance in fate of individual naïve T cells argues against asymme..View full abstract
Awarded by Walter-Hitzig fellowship from the CCI
Awarded by Deutsche Forschungsgemeinschaft
Awarded by Nederlandse Organisatie voor Wetenschappelijk Onderzoek Veni grant
The authors thank A. Pfauth and F. van Diepen for cell sorting; E. Borg for technical assistance; D. Zehn for provision of LM-OVA (T4); S. Harkema for helpful discussions; and G. Bendle, M. C. Wolkers, and E. A. Moseman for critically reading the manuscript. The data presented in this manuscript are tabulated in the main paper and in the supplementary materials. The authors declare no conflict of interest. This research was funded by European Research Council AdG Life-his-T to T.N.M.S., a Walter-Hitzig fellowship from the CCI (BMBF 01EO0803), a Deutsche Forschungsgemeinschaft (DFG RO 4120/1-1) research fellowship to J.C.R., a Marie Curie Intra European Fellowship to L. P., a Nederlandse Organisatie voor Wetenschappelijk Onderzoek Veni grant (916.86.080) to J.B.B., and a Leukemia and Lymphoma Society Career Development Fellowship to S.H.N.