Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients
Peng-Yuan Wang, Lauren R Clements, Helmut Thissen, Wei-Bor Tsai, Nicolas H Voelcker
Acta Biomaterialia | ELSEVIER SCI LTD | Published : 2015
It is well known that the surface chemistry of biomaterials is important for both initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high throughput. In this study, two surface chemical gradients were fabricated using diffusion control during plasma polymerization via a tilted mask. Acrylic acid (AA) plasma polymer gradients were coated on a uniform 1,7-octadiene (OD) plasma polymer layer to generate OD-AA plasma polymer gradients, whilst diethylene glycol dimethyl ether (DG) plasma polymer gradients were coated on a uniform AA plasma polymer layer to generate AA-DG..View full abstract
Awarded by National Science Council (Taiwan) via the award of a travel scholarship under the Graduate Program for Studying (GPS) in Australia/New Zealand scheme
The authors gratefully acknowledge funding from the Australian Research Council. P.Y.W. acknowledges support from the National Science Council (Taiwan) via the award of a travel scholarship under the Graduate Program for Studying (GPS) in Australia/New Zealand scheme (98-2911-I-002-056). L.C. acknowledges support from the CSIRO Office of the Chief Executive (OCE). We are grateful to Marta Redrado Notivoli from the Materials Characterisation and Fabrication Platform, University of Melbourne, for her assistance with AFM measurements.