Characterization of MHC class IA in the endangered southern corroboree frog

TA Kosch; JA Eimes; C Didinger; LA Brannelly; B Waldman; L Berger; LF Skerratt

Journal article

Characterization of MHC class IA in the endangered southern corroboree frog

TA Kosch, JA Eimes, C Didinger, LA Brannelly, B Waldman, L Berger, LF Skerratt

Immunogenetics | SPRINGER | Published : 2017

DOI: 10.1007/s00251-016-0965-3

Abstract

Southern corroboree frogs (Pseudophryne corroboree) have declined to near extinction in the wild after the emergence of the amphibian chytrid fungus Batrachochytrium dendrobatidis in southeastern Australia in the 1980s. A major captive breeding and reintroduction program is underway to preserve this iconic species, but improving resistance to B. dendrobatidis would help the wild population to be self-sustaining. Using 3′ and 5′ rapid amplification of complementary DNA ends (RACE), we characterized the major histocompatibility complex (MHC) class IA locus in this species. We then used sequences generated from RACE to design primers to amplify the peptide-binding region (PBR) of this functiona..

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University of Melbourne Researchers

Tiffany Kosch Author

Laura Brannelly Author

Lee Berger Author

Lee Skerratt Author

Grants

Awarded by Australian Research Council

Funding Acknowledgements

We thank Gerry Marantelli of the Amphibian Research Centre for providing the P. corroboree used in this study. Funding was provided by the Australian Research Council grants LP110200240 and FT100100375, the National Research Foundation of Korea grant 2015R1D1A1A01057282 (to B.W.) funded by the government of the Republic of Korea (MOE), the Taronga Conservation Society, and the New South Wales Office of Environment and Heritage.