Journal article
Isolation of contractile cardiomyocytes from human pluripotent stem-cell-derived cardiomyogenic cultures using a human NCX1-EGFP reporter
DA Ovchinnikov, A Hidalgo, SK Yang, X Zhang, J Hudson, SB Mazzone, C Chen, JJ Cooper-White, EJ Wolvetang
Stem Cells and Development | MARY ANN LIEBERT, INC | Published : 2015
Abstract
The prospective isolation of defined contractile human pluripotent stem cell (hPSC)-derived cardiomyocytes is advantageous for regenerative medicine and drug screening applications. Currently, enrichment of cardiomyocyte populations from such cultures can be achieved by combinations of cell surface markers or the labor-intensive genetic modification of cardiac developmental genes, such as NKX2.5 or MYH6, with fluorescent reporters. To create a facile, portable method for the isolation of contractile cardiomyocytes from cardiomyogenic hPSC cultures, we employed a highly conserved cardiac enhancer sequence in the SLC8A1 (NCX1) gene to generate a lentivirally deliverable, antibiotic-selectable ..
View full abstractGrants
Funding Acknowledgements
The authors would like to thank the StemCore facility at the UQ AIBN for the provision of hES cell line (H9) and logistical support in karyotyping analyses. We would also like to acknowledge the Australian Stem Cell Centre and the ARC Excellence Centre "Stem Cells Australia" for the funding support.