Journal article
Study of gap junctions in human embryonic stem cells
A Pébay, RCB Wong
Methods in Molecular Biology | HUMANA PRESS INC | Published : 2014
DOI: 10.1007/7651_2014_83
Abstract
Gap junctional intercellular communication (GJIC) has been described in different cell types including stem cells and has been involved in different biological events. GJIC is required for mouse embryonic stem cell maintenance and proliferation and various studies suggest that functional GJIC is a common characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap junctions in hESC, from expression of gap junction proteins to functional study of GJIC in hESC proliferation, apoptosis, colony growth, and pluripotency.
Grants
Awarded by NHMRC
Funding Acknowledgements
This work was supported by a NHMRC Career Development Award Fellowship (AP) and project grant (#454723), the Cranbourne Foundation (RCBW), the University of Melbourne and the National Stem Cell Foundation of Australia. CERA receives infrastructure support from the Victorian State Government's Department of Innovation, Industry and Regional Development.