Journal article
Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): Results from an international ring trial targeting four species of the subgenus Avaritia
C Garros, T Balenghien, S Carpenter, JC Delécolle, R Meiswinkel, A Pédarrieu, I Rakotoarivony, L Gardès, N Golding, J Barber, M Miranda, DB Borràs, M Goffredo, F Monaco, N Pagès, S Sghaier, S Hammami, JH Calvo, J Lucientes, D Geysen Show all
Parasites and Vectors | BMC | Published : 2014
Abstract
Background: Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly. Methods. In this study, designed as an inter-laboratory ring trial with twelve partners from Europe and North Africa, we assess four PCR-based assays which are used routinely to differentiate the fo..
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Awarded by Biotechnology and Biological Sciences Research Council
Funding Acknowledgements
This work was funded by the MedReoNet Project No. 044285 from the Sixth Framework Programme of the European Commission, SSPE-CT-2006-044285. We thank the two anonymous reviewers for their useful comments.