Validation of method for enhanced production of red-shifted bioluminescent photons in vivo
Joe Dragavon, Samantha Blazquez, Kelly L Rogers, Chelsea Samson, Regis Tournebize, Spencer Shorte, DL Farkas (ed.), DV Nicolau (ed.), RC Leif (ed.)
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES IX | SPIE-INT SOC OPTICAL ENGINEERING | Published : 2011
Bioluminescence Imaging (BLI) is an increasingly useful and applicable technique that allows for the non-invasive observation of biological events in intact living organisms, ranging from single cells to small rodents. Though the photon production occurs within the host, significant exposure times can be necessary due to the low photon flux compared to fluorescence imaging. The optical absorption spectrum of haemoglobin strongly overlaps most bioluminescent emission spectra, greatly attenuating the total detectable photons in animal models. We have developed and validated a technique that is able to red-shift the bioluminescent photons to the more desirable optical region of > 650 nm, a regi..View full abstract
The authors would like to thank the Institut Carnot Pasteur Maladies Infectieuses and the PasteurUS Foundation for financial support.