Journal article
New device for real-time bioluminescence imaging in moving rodents
E Roncali, M Savinaud, O Levrey, KL Rogers, S Maitrejean, B Tavitian
Journal of Biomedical Optics | SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS | Published : 2008
DOI: 10.1117/1.2976426
Abstract
Bioluminescence imaging (BLI) allows detection of biological functions in genetically modified cells, bacteria, or animals expressing a luciferase (i.e., firefly, Renilla, or aequorin). Given the high sensitivity and minimal toxicity of BLI, in vivo studies on molecular events can be performed noninvasively in living rodents. To date, detection of bioluminescence in living animals has required long exposure times that are incompatible with studies on dynamic signaling pathways or nonanaesthetised freely moving animals. Here we develop an imaging system that allows: 1. bioluminescence to be recorded at a rate of 25 images/s using a third generation intensified charge-coupled device (CCD) came..
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Funding Acknowledgements
The authors would like to acknowledge the contributions of Raphael Boisgard, Frederic Duconge, Karine Gombert, Carine Pestourie, and Thomas Viel (CEA-LIME and INSERM U803, Paris, France) for in vitro and in vivo experiments, and Philippe Brulet (Institut Pasteur, Paris, France) for providing the GA mice. Roncali and Savinaud have a scholarship from Biospace Lab. This work was supported by the EMIL and DIMI European Networks to Tavitian.