Journal article

Fluorescence detection of plant extracts that affect neuronal voltage-gated Ca2 channels

KL Rogers, WF Fong, J Redburn, LR Griffiths

European Journal of Pharmaceutical Sciences | ELSEVIER SCIENCE BV | Published : 2002

Abstract

Structurally novel compounds able to block voltage-gated Ca2+ channels (VGCCs) are currently being sought for the development of new drugs directed at neurological disorders. Fluorescence techniques have recently been developed to facilitate the analysis of VGCC blockers in a multi-well format. By utilising the small cell lung carcinoma cell line, NCI-H146, we were able to detect changes in intracellular Ca2+ concentration ([Ca2+](i)) using a fluorescence microplate reader. NCI-H146 cells have characteristics resembling those of neuronal cells and express multiple VGCC subtypes, including those of the L-, N- and P-type. We found that K+-depolarisation of fluo-3 loaded NCI-H146 cells causes a..

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University of Melbourne Researchers