Journal article

A QCM-D and SAXS Study of the Interaction of Functionalised Lyotropic Liquid Crystalline Lipid Nanoparticles with siRNA

Behnoosh Tajik-Ahmadabad, Adam Mechler, Benjamin W Muir, Keith McLean, Tracey M Hinton, Frances Separovic, Anastasios Polyzos

ChemBioChem | Wiley | Published : 2017

Abstract

Biophysical studies were undertaken to investigate the binding and release of short interfering ribonucleic acid (siRNA) from lyotropic liquid crystalline lipid nanoparticles (LNPs) by using a quartz crystal microbalance (QCM). These carriers are based on phytantriol (Phy) and the cationic lipid DOTAP (1,2‐dioleoyloxy‐3‐(trimethylammonium)propane). The nonlamellar phase LNPs were tethered to the surface of the QCM chip for analysis based on biotin–neutravidin binding, which enabled the controlled deposition of siRNA–LNP complexes with different lipid/siRNA charge ratios on a QCM‐D crystal sensor. The binding and release of biomolecules such as siRNA from LNPs was demonstrated to be reliably ..

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