Journal article

Orientation and Location of the Cyclotide Kalata B1 in Lipid Bilayers Revealed by Solid-State NMR

Stephan L Grage, Marc-Antoine Sani, Olivier Cheneval, Sonia Troeira Henriques, Constantin Schalck, Ralf Heinzmann, Joshua S Mylne, Pavel K Mykhailiuk, Sergii Afonin, Igor V Komarov, Frances Separovic, David J Craik, Anne S Ulrich

Biophysical Journal | Cell Press | Published : 2017

Abstract

Cyclotides are ultra-stable cyclic disulfide-rich peptides from plants. Their biophysical effects and medically interesting activities are related to their membrane-binding properties, with particularly high affinity for phosphatidylethanolamine lipids. In this study we were interested in understanding the molecular details of cyclotide-membrane interactions, specifically with regard to the spatial orientation of the cyclotide kalata B1 from Oldenlandia affinis when embedded in a lipid bilayer. Our experimental approach was based on the use of solid-state 19F-NMR of oriented bilayers in conjunction with the conformationally restricted amino acid L-3-(trifluoromethyl)bicyclopent-[1.1.1]-1-ylg..

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Grants

Awarded by National Health and Medical Research Council (NHMRC)


Awarded by Australian Research Council (ARC)


Awarded by Australian Research Council


Funding Acknowledgements

We gratefully acknowledge the Helmholtz Association (program Biointerfaces in Technology and Medicine) and the National Health and Medical Research Council (NHMRC) project grant (APP1084965) for financial support. S.T.H. was supported by an Australian Research Council (ARC) Discovery Early Career Research Award (DE120103152). D.J.C. is an ARC Laureate Fellow (FL150100146).