Journal article

Data Descriptor: Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology

Steven P Williams, Cathryn M Gould, Cameron J Nowell, Tara Karnezis, Marc G Achen, Kaylene J Simpson, Steven A Stacker



Many cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content m..

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Funding Acknowledgements

This work was funded partly by a Program Grant from the National Health and Medical Research Council of Australia (NH&MRC). S.A.S. and M.G.A. are supported by Senior Research Fellowships from the NH&MRC. S.A.S. would like to acknowledge the support of the Pfizer Australia Fellowship. S.P.W. received a Doctoral Research Scholarship from the National Breast Cancer Foundation. We thank members of the Tumour Angiogenesis Program for helpful discussions. We wish to thank Daniel Thomas and Yanny Handoko for screening assistance and Piyush Madhamshettiwar for managing the PubChem submissions (Victorian Centre for Functional Genomics-VCFG). The VCFG is funded by the Australian Cancer Research Foundation (ACRF), the Victorian Department of Industry, Innovation and Regional Development (DIIRD), the Australian Phenomics Network supported by funding from the Australian Government's Education Investment Fund through the Super Science Initiative, the Australasian Genomics Technologies Association, the Brockhoff Foundation and the Peter MacCallum Foundation.