Journal article

A forward genetic screen identifies a negative regulator of rapid Ca2 -dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

James M McCoy, Rebecca J Stewart, Alessandro D Uboldi, Dongdi Li, Jan Schroder, Nicollas E Scott, Anthony T Papenfuss, Adele M Lehane, Leonard J Foster, Christopher J Tonkin

JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2017

Abstract

Toxoplasma gondii, like all apicomplexan parasites, uses Ca2+ signaling pathways to activate gliding motility to power tissue dissemination and host cell invasion and egress. A group of "plant-like" Ca2+-dependent protein kinases (CDPKs) transduces cytosolic Ca2+ flux into enzymatic activity, but how they function is poorly understood. To investigate how Ca2+ signaling activates egress through CDPKs, we performed a forward genetic screen to isolate gain-of-function mutants from an egress-deficient cdpk3 knockout strain. We recovered mutants that regained the ability to egress from host cells that harbored mutations in the gene Suppressor of Ca2+-dependent Egress 1 (SCE1). Global phosphoprote..

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