Legionella pneumophila Strain 130b Evades Macrophage Cell Death Independent of the Effector SidF in the Absence of Flagellin
Mary Speir, Adam Vogrin, Azadeh Seidi, Gilu Abraham, Stephane Hunot, Qingqing Han, Gerald W Dorn, Seth L Masters, Richard A Flavell, James E Vince, Thomas Naderer
Frontiers in Cellular and Infection Microbiology | FRONTIERS MEDIA SA | Published : 2017
The human pathogen Legionella pneumophila must evade host cell death signaling to enable replication in lung macrophages and to cause disease. After bacterial growth, however, L. pneumophila is thought to induce apoptosis during egress from macrophages. The bacterial effector protein, SidF, has been shown to control host cell survival and death by inhibiting pro-apoptotic BNIP3 and BCL-RAMBO signaling. Using live-cell imaging to follow the L. pneumophila-macrophage interaction, we now demonstrate that L. pneumophila evades host cell apoptosis independent of SidF. In the absence of SidF, L. pneumophila was able to replicate, cause loss of mitochondria membrane potential, kill macrophages, and..View full abstract
Awarded by National Health and Medical Research Council (Canberra, Australia)
Awarded by CDF1 Fellowship
This project was funded by the National Health and Medical Research Council (Canberra, Australia) as part of the Project Grant #1024839 (JV and TN) and CDF1 Fellowship #1052598 JV).