Journal article

PURINERGIC P2 RECEPTORS IN THE PARAVENTRICULAR NUCLEUS OF THE HYPOTHALAMUS ARE INVOLVED IN HYPEROSMOTIC-INDUCED SYMPATHOEXCITATION

HC Ferreira-Neto, IMR Ribeiro, TS Moreira, ST Yao, VR Antunes

Neuroscience | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017

Abstract

Increases in plasma osmolality activates the paraventricular nucleus of the hypothalamus (PVN) which in turn mounts a physiological response by increasing the release of arginine vasopressin and sympathetic nerve activity to end organs such as the kidney. The PVN expresses an abundance of purinergic receptors including P2X2 receptors. In the present study, we sought to determine (1) whether P2X2-expressing PVN neurons are activated by hypertonic saline or hypertonic mannitol and (2) what effects P2X receptor blockade has on sympathetic nerve activation mediated by a hyperosmotic stimulus. Male Wistar rats were randomly assigned to three groups and intravenously infused with either isotonic s..

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University of Melbourne Researchers

Grants

Awarded by Sao Paulo Research Foundation (FAPESP)


Awarded by FAPESP


Awarded by National Council for Scientific and Technological Development (CNPq)


Awarded by National Health and Medical Research Council (NHMRC, Australia)


Funding Acknowledgements

We thank Dr Patricia Castelucci for the generous gift of the guinea pig anti-P2X2 receptor antibody. This study was supported by the Sao Paulo Research Foundation (FAPESP); #2016/21991-3, #13/06206-0, #10/17997-0 and National Council for Scientific and Technological Development (CNPq). HCFN was a recipient of a FAPESP fellowship #10/05037-1. IMRR was a recipient of the National Council for Scientific and Technological Development (CNPq) fellowship #141929/2012-0. STY is supported by the National Health and Medical Research Council (NHMRC, Australia, GNT1050268; GNT1079680) and the Victorian Government through the Operational Infrastructure Scheme.