Measuring macromolecular crowding in cells through fluorescence anisotropy imaging with an AIE fluorogene
Hamid Soleimaninejad, Moore Z Chen, Xiaoding Lou, Trevor A Smith, Yuning Hong
Chemical Communications | Royal Society of Chemistry | Published : 2017
We report a new strategy that allows spatiotemporal visualization of the macromolecular crowding effect in cells. An amine-reactive aggregation-induced emission fluorogen is used to label proteins in the cytoplasm and the change in the protein mobility as well as local viscosity can be monitored by using fluorescence anisotropy imaging and fluorescence lifetime imaging, respectively.
We thank A/Prof. Danny Hatters from the University of Melbourne for providing access to the tissue culture facility and cell lines. We also thank Peng Zeng from the University of Melbourne for sharing the MatLab code for image analysis. Y. H. thanks the support from the McKenzie Fellowship of The University of Melbourne and Bruce Stone Fellowship of La Trobe University.