Journal article

Sialic acid-to-urea ratio as a measure of airway surface hydration

Charles R Esther, David B Hill, Brian Button, Shuai Shi, Corey Jania, Elizabeth A Duncan, Claire M Doerschuk, Gang Chen, Sarath Ranganathan, Stephen M Stick, Richard C Boucher

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2017

Abstract

Although airway mucus dehydration is key to pathophysiology of cystic fibrosis (CF) and other airways diseases, measuring mucus hydration is challenging. We explored a robust method to estimate mucus hydration using sialic acid as a marker for mucin content. Terminal sialic acid residues from mucins were cleaved by acid hydrolysis from airway samples, and concentrations of sialic acid, urea, and other biomarkers were analyzed by mass spectrometry. In mucins purified from human airway epithelial (HAE), sialic acid concentrations after acid hydrolysis correlated with mucin concentrations (r2 = 0.92). Sialic acid-to-urea ratios measured from filters applied to the apical surface of cultured HAE..

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Grants

Awarded by NIH/NHLBI


Awarded by NC TraCS


Awarded by NIH/NIEHS


Awarded by NHMRC


Awarded by CFF


Funding Acknowledgements

C. R. Esther and D. B. Hill were supported by NIH/NHLBI R01-HL116228. C. R. Esther was also supported by NC TraCS 50KR51009, NIH/NIEHS P30-ES10126, and NIH/NHLBI K23-HL089708. C. Jania, E. A. Duncan, and C. M. Doerschuk were supported by NIH/NHLBI P50 HL120100. S. Ranganathan and S.M. Stick were supported by NHMRC GNT1000896. B. Button and R. C. Boucher were supported by NIH/NHLBI HL34322, HL107168, P01-HL08808, P30-DK065988, P01-HL110873, and P50-HL107168. Research reported in this publication was supported by the National Heart, Lung, and Blood Institute (NHLBI) and Food and Drug Administration (FDA) Center for Tobacco Products. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH or the FDA. B. Button was supported by NIH/NHLBI R01-HL-125280 and CFF BUTTON07XXO.