Journal article
BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis in Arabidopsis by phosphorylating cellulose synthase 1
C Sánchez-Rodríguez, KD Ketelaar, R Schneider, JA Villalobos, CR Somerville, S Persson, IS Wallace
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2017
Abstract
The deposition of cellulose is a defining aspect of plant growth and development, but regulation of this process is poorly understood. Here, we demonstrate that the protein kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), a key negative regulator of brassinosteroid (BR) signaling, can phosphorylate Arabidopsis cellulose synthase A1 (CESA1), a subunit of the primary cell wall cellulose synthase complex, and thereby negatively regulate cellulose biosynthesis. Accordingly, point mutations of the BIN2-mediated CESA1 phosphorylation site abolished BIN2-dependent regulation of cellulose synthase activity. Hence,we have uncovered a mechanism for how BR signaling can modulate cellulose synthesis in plant..
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Awarded by National Science Foundation
Funding Acknowledgements
We thank Dr. Christopher Kesten and Dr. Edwin Lampugnani for experimental assistance and critical evaluation of this manuscript. C.S.-R. and S.P. were financially supported by the Max-Planck Gesellschaft. S.P. was supported by a R@MAP Professorship at the University of Melbourne. K.K. and I.S.W. are supported by startup funds from the Biochemistry and Molecular Biology Department at the University of Nevada, Reno as well as the National Science Foundation (Grant IOS 1449068) and a Nevada Agricultural Experiment Station Award (NEV00382). Support was also received from the Energy Biosciences Institute at the University of California, Berkeley and the Philomathia Foundation.