Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells
Wen Juan Tu, Kristine Hardy, Christopher R Sutton, Robert McCuaig, Jasmine Li, Jenny Dunn, Abel Tan, Vedran Brezar, Melanie Morris, Gareth Denyer, Sau Kuen Lee, Stephen J Turner, Nabila Seddiki, Corey Smith, Rajiv Khanna, Sudha Rao
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2017
Memory T cells exhibit transcriptional memory and "remember" their previous pathogenic encounter to increase transcription on re-infection. However, how this transcriptional priming response is regulated is unknown. Here we performed global FAIRE-seq profiling of chromatin accessibility in a human T cell transcriptional memory model. Primary activation induced persistent accessibility changes, and secondary activation induced secondary-specific opening of previously less accessible regions associated with enhanced expression of memory-responsive genes. Increased accessibility occurred largely in distal regulatory regions and was associated with increased histone acetylation and relative H3.3..View full abstract
Awarded by NHMRC
Awarded by ARC
This research was supported by grants from the NHMRC (GNT1105747), ARC (DP1401579) and a University of Canberra Early Career Research Grant. WJT was supported by a WJ Weeden Research Scholarship. SJT is supported by a NHMRC Principal Research Fellowship.