Analysis of variability in partial sequences of genomes of Tobacco yellow dwarf virus isolates

Abstract

The movement protein coding region of six isolates of Tobacco yellow dwarf virus (TYDV), collected over a 30 year period from various regions throughout Australia, was analysed using PCR and DNA sequencing. Four isolates of Bean summer death virus, considered a synonym of TYDV, were also analysed. Phylogenetic analysis showed that all 10 isolates were greater than 95% homologous to the published TYDV sequence (GenBank accession number M81103). The weed Raphanus raphanistrum was identified as an alternative host of TYDV for the first time.