Analysis of the human monocyte-derived macrophage transcriptome and response to lipopolysaccharide provides new insights into genetic aetiology of inflammatory bowel disease

JK Baillie; E Arner; C Daub; M De Hoon; M Itoh; H Kawaji; T Lassmann; P Carninci; ARR Forrest; Y Hayashizaki; GJ Faulkner; CA Wells; M Rehli; P Pavli; KM Summers; DA Hume

Journal article

Analysis of the human monocyte-derived macrophage transcriptome and response to lipopolysaccharide provides new insights into genetic aetiology of inflammatory bowel disease

JK Baillie, E Arner, C Daub, M De Hoon, M Itoh, H Kawaji, T Lassmann, P Carninci, ARR Forrest, Y Hayashizaki, GJ Faulkner, CA Wells, M Rehli, P Pavli, KM Summers, DA Hume

Plos Genetics | Published : 2017

DOI: 10.1371/journal.pgen.1006641

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Abstract

The FANTOM5 consortium utilised cap analysis of gene expression (CAGE) to provide an unprecedented insight into transcriptional regulation in human cells and tissues. In the current study, we have used CAGE-based transcriptional profiling on an extended dense time course of the response of human monocyte-derived macrophages grown in macrophage colony-stimulating factor (CSF1) to bacterial lipopolysaccharide (LPS). We propose that this system provides a model for the differentiation and adaptation of monocytes entering the intestinal lamina propria. The response to LPS is shown to be a cascade of successive waves of transient gene expression extending over at least 48 hours, with hundreds of ..

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University of Melbourne Researchers

Christine Wells Author

Grants

Awarded by Biotechnology and Biological Sciences Research Council

Funding Acknowledgements

This study was supported by an Institute Strategic Programme Grants (BB/G004013/1,BB/ I025328/1,BBS/E/D/20211550) from the Biotechnology and Biological Sciences Research CounciltoThe Roslin Institute. JKB is funded by a Wellcome-Beit Prize Intermediate Clinical Fellowship(103258/Z/13/Z,A) and the UK Intensive Care Foundation. FANTOM5 was made possible by a Research Grant for RIKEN Omics Science Center from MEXT to YH and a grant of the Innovative Cell Biology by Innovative Technology (CellInnovation Program) from the MEXT, Japan to YH. It was also supported by Research Grants for RIKEN Preventive Medicine and Diagnosis Innovation Program to YH and RIKEN Centre for Life Science Technologies, Division of Genomic Technologies (from the MEXT,Japan). The funders had no role in study design,data collection and analysis, decision to publish,orpreparation of the manuscript.