Plasmodium falciparum Transfected with Ultra Bright NanoLuc Luciferase Offers High Sensitivity Detection for the Screening of Growth and Cellular Trafficking Inhibitors
Mauro F Azevedo, Catherine Q Nie, Brendan Elsworth, Sarah C Charnaud, Paul R Sanders, Brendan S Crabb, Paul R Gilson
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2014
Drug discovery is a key part of malaria control and eradication strategies, and could benefit from sensitive and affordable assays to quantify parasite growth and to help identify the targets of potential anti-malarial compounds. Bioluminescence, achieved through expression of exogenous luciferases, is a powerful tool that has been applied in studies of several aspects of parasite biology and high throughput growth assays. We have expressed the new reporter NanoLuc (Nluc) luciferase in Plasmodium falciparum and showed it is at least 100 times brighter than the commonly used firefly luciferase. Nluc brightness was explored as a means to achieve a growth assay with higher sensitivity and lower..View full abstract
Awarded by National Health and Medical Research Council
This work was supported by the National Health and Medical Research Council Grants 637406, APP1068287 and APP1021560 awarded to BSC and PRG (URL:www.nhmrc.gov.au). The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.