Journal article

Internal ribosomal entry site-mediated translation initiation in equine rhinitis A virus: Similarities to and differences from that of foot-and-mouth disease virus

TM Hinton, F Li, BS Crabb

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2000


Equine rhinitis A virus (ERAV) has recently been classified as an aphthovirus, a genus otherwise comprised of the different serotypes of Foot-and-mouth disease virus (FMDV). FMDV initiates translation via a type II internal ribosomal entry site (IRES) and utilizes two in-frame AUG codons to produce the leader proteinases Lab and Lb. Here we show that the ERAV 5' nontranslated region also possesses the core structures of a type II IRES. The functional activity of this region was characterized by transfection of bicistronic plasmids into BHK-21 cells. In this system the core type II structures, stem-loops D to L, in addition to a stem-loop (termed M) downstream of the first putative initiation..

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University of Melbourne Researchers