Journal article

Bcl-w is an important determinant of damage-induced apoptosis in epithelia of small and large intestine

DM Pritchard, C Print, L O'Reilly, JM Adams, CS Potten, JA Hickman

Oncogene | NATURE PUBLISHING GROUP | Published : 2000

Abstract

The potential role of the bcl-2 relative bcl-w as a physiological regulator of apoptosis in intestinal epithelia has been investigated. Immunoblots for bcl-w with new monoclonal antibodies revealed that it was expressed in the small intestine and colon, among other murine tissues, as well as in six human tumour cell lines of epithelial origin, including two colon carcinoma lines. To assess whether bcl-w regulates either spontaneous or damage-induced apoptosis in the small intestine or colon, apoptosis in intestinal crypts of bcl-w -/- and wild-type mice was quantified microscopically on a cell positional basis. Spontaneous apoptosis within crypt epithelia was not significantly increased by l..

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