Generation of altered transcripts by retroviral insertion within the c-myb gene in two murine monocytic leukemias

Abstract

Two murine monocytic leukemia cell lines, WEHI-265 and WEHI-274, were found to carry a rearranged c-myb gene. The rearrangements are due to insertion of a deleted Moloney murine leukemia virus (Mo-MLV) provirus in the 5' region of the c-myb gene and thus are similar to rearrangements in the ABPL tumors (G.L.C. Shen-Ong, M. Potter, J.F. Mushinski, S. Lavu, E.P. Reddy, Science 226:1077-1080, 1984). In each cell line, the retroviral insertion has induced high levels of two aberrant RNA species, which, as in the ABPL tumors (G.L.C. Shen-Ong, H.C. Morse, M. Potter, and J.F. Mushinski, Mol. Cell. Biol. 6:380-392, 1986), contain both viral (Mo-MLV) and cellular (myb) sequences. Both species lack th..