Journal article

PLASMACYTOMAS WITH MORE THAN ONE IMMUNOGLOBULIN KAPPA-MESSENGER RNA - IMPLICATIONS FOR ALLELIC EXCLUSION

O BERNARD, NM GOUGH, JM ADAMS

Proceedings of the National Academy of Sciences | NATL ACAD SCIENCES | Published : 1981

Abstract

Although only one allele of an immunoglobulin gene is thought to be expressed as a polypeptide by a given lymphocyte ("allelic exclusion"), three murine plasmacytomas were found to contain more than one kappa light chain mRNA species, as evidenced by the sequences of distinct kappa cDNA clones. Two different kappa cDNA sequences were cloned from BFPC 61 microsomal mRNA, two from MOPC 173, and three from S107. One cDNA sequence from each tumor matches the known secreted polypeptide, while the variant sequences differ in the variable (V) region. Hence fusion of a V kappa gene to a joining (J kappa) gene has occurred independently on separate homologous chromosomes and each allele is transcript..

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University of Melbourne Researchers