Reproduction and maternal behavior in insulin-regulated aminopeptidase (IRAP) knockout mice
Vi Pham, Peta Burns, Anthony L Albiston, Holly R Yeatman, Leelee Ng, Shand Diwakarla, Siew Yeen Chai
PEPTIDES | ELSEVIER SCIENCE INC | Published : 2009
During human pregnancy, a circulating form of insulin-regulated aminopeptidase (IRAP EC 220.127.116.11), often termed oxytocinase or placental leucine aminopeptidase (PLAP), is present in plasma. It is proposed that circulating IRAP plays an important role in regulating the circulating levels of oxytocin and/or vasopressin during pregnancy. We assessed the reproductive and maternal profile of global IRAP knock out mice. No differences in the reproductive profile were observed, with normal gestational period, litter size and parturition recorded. However, western blot analysis of pregnant mouse serum, failed to detect IRAP, a result which was confirmed by fluorimetric IRAP enzyme assay. A review of..View full abstract
This research was supported by the Robert J. Kleberg Jr. and Helen C. Kleberg Foundation and the Cass Foundation of Australia. SYC is a National Health and Medical Research Council (NHMRC) Senior Research Fellow and V.P. is an NHMRC Peter Doherty fellow.