Journal article
The 2'-O-methyladenosine nucleoside modification gene OsTRM13 positively regulates salt stress tolerance in rice
Youmei Wang, Dongqin Li, Junbao Gao, Xukai Li, Rui Zhang, Xiaohuan Jin, Zhen Hu, Bo Zheng, Staffan Persson, Peng Chen
Journal of Experimental Botany | Oxford University Press (OUP) | Published : 2017
DOI: 10.1093/jxb/erx061
Abstract
Stress induces changes of modified nucleosides in tRNA, and these changes can influence codon–anticodon interaction and therefore the translation of target proteins. Certain nucleoside modification genes are associated with regulation of stress tolerance and immune response in plants. In this study, we found a dramatic increase of 2′-O-methyladenosine (Am) nucleoside in rice seedlings subjected to salt stress and abscisic acid (ABA) treatment. We identified LOC_Os03g61750 (OsTRM13) as a rice candidate methyltransferase for the Am modification. OsTRM13 transcript levels increased significantly upon salt stress and ABA treatment, and the OsTrm13 protein was found to be located primarily to the..
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Awarded by National Natural Science Foundation of China
Awarded by Fundamental Research Funds for the Central Universities Project
Funding Acknowledgements
This work was supported by the National Natural Science Foundation of China (31100268 to PC, and 31370604 to BZ); the Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry), and Fundamental Research Funds for the Central Universities Project (2662015PY168). SP was supported by a R@MAP Professorship at the University of Melbourne. We are grateful to Gunilla Jager (Department of Molecular Biology, Umea University, Sweden) for technical help in nucleoside analysis. We thank Prof. Glenn Bjork (Umea University) for the yeast strains for complementation.