Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA
Dmitriy A Khodakov, Anastasia S Khodakova, David M Huang, Adrian Linacre, Amanda V Ellis
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2015
Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement react..View full abstract
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Awarded by Australian Research Council Future Fellowship Fund
The authors kindly acknowledge the Australian Research Council Future Fellowship Fund (Grant No. FT130100211) for funding.