Journal article

Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA

Dmitriy A Khodakov, Anastasia S Khodakova, David M Huang, Adrian Linacre, Amanda V Ellis

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2015

Abstract

Single nucleotide polymorphisms (SNPs) are a prime source of genetic diversity. Discriminating between different SNPs provides an enormous leap towards the better understanding of the uniqueness of biological systems. Here we report on a new approach for SNP discrimination using toehold-mediated DNA strand displacement. The distinctiveness of the approach is based on the combination of both 3- and 4-way branch migration mechanisms, which allows for reliable discrimination of SNPs within double-stranded DNA generated from real-life human mitochondrial DNA samples. Aside from the potential diagnostic value, the current study represents an additional way to control the strand displacement react..

View full abstract

University of Melbourne Researchers