Journal article

A DNA Circuit for IsomiR Detection

Ashley R Connolly, Rena Hirani, Amanda V Ellis, Matt Trau

CHEMBIOCHEM | WILEY-V C H VERLAG GMBH | Published : 2016

Abstract

A synthetic DNA oligonucleotide has been programmed to function as a biological circuit to detect 5'-IsomiRs. The circuit consists of two integrated DNA switches. The first is "activated" when a DNA probe is enzymatically modified by a reverse transcriptase that incorporates nucleotides complementary to the 5'-region of a microRNA (miRNA). Addition of the correct number and sequence of nucleotides enables the probe to assemble into an asymmetric DNA hairpin. The reconfigured hairpin probe then primes an internal polymerisation reaction, resulting in the synthesis of a symmetrical DNA hairpin. This activates the second switch, which then initiates the amplification of reverse-transcribed miRN..

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University of Melbourne Researchers