A DNA Circuit for IsomiR Detection
Ashley R Connolly, Rena Hirani, Amanda V Ellis, Matt Trau
CHEMBIOCHEM | WILEY-V C H VERLAG GMBH | Published : 2016
A synthetic DNA oligonucleotide has been programmed to function as a biological circuit to detect 5'-IsomiRs. The circuit consists of two integrated DNA switches. The first is "activated" when a DNA probe is enzymatically modified by a reverse transcriptase that incorporates nucleotides complementary to the 5'-region of a microRNA (miRNA). Addition of the correct number and sequence of nucleotides enables the probe to assemble into an asymmetric DNA hairpin. The reconfigured hairpin probe then primes an internal polymerisation reaction, resulting in the synthesis of a symmetrical DNA hairpin. This activates the second switch, which then initiates the amplification of reverse-transcribed miRN..View full abstract
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Awarded by National Health and Medical Research Council
Awarded by Australian Research Council
This work is supported by the National Breast Cancer Foundation (NBCF) program and project grants, National Health and Medical Research Council (NHMRC1029579) project grant and the Australian Research Council: Future Fellowship Grant no. FT130100211.