Journal article

Development and application of a TaqMan single nucleotide polymorphism genotyping assay to study infectious laryngotracheitis virus recombination in the natural host

Carlos A Loncoman, Carol A Hartley, Mauricio JC Coppo, Paola K Vaz, Andres Diaz-Mendez, Glenn F Browning, Sang-won Lee, Joanne M Devlin

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2017

Abstract

To date, recombination between different strains of the avian alphaherpesvirus infectious laryngotracheitis virus (ILTV) has only been detected in field samples using full genome sequencing and sequence analysis. These previous studies have revealed that natural recombination is widespread in ILTV and have demonstrated that recombination between two attenuated ILTV vaccine strains generated highly virulent viruses that produced widespread disease within poultry flocks in Australia. In order to better understand ILTV recombination, this study developed a TaqMan single nucleotide polymorphism (SNP) genotyping assay to detect recombination between two field strains of ILTV (CSW-1 and V1-99 ILTV..

View full abstract

Grants

Awarded by Australian Research Council


Funding Acknowledgements

This work was supported by the Australian Research Council (FT140101287). Carlos Loncoman is supported by Becas Chile, CONICYT, Gobierno de Chile.We gratefully acknowledge Mahathi Nadimpalli, Nino Ficorilli and Mesula Korsa for their help with cell culture, Jose Quinteros, June Daly, Jenece Wheeler and Cheryl Colson for their help with the in vivo studies, and Zelalem Mekuria and Alistair Legione for their help with the Geneious software. This work was supported by the Australian Research Council (FT140101287). Carlos Loncoman is supported by Becas Chile, CONICYT, Gobierno de Chile.