Journal article
DNA methylation changes at infertility genes in newborn twins conceived by in vitro fertilisation
JE Castillo-Fernandez, YJ Loke, S Bass-Stringer, F Gao, Y Xia, H Wu, H Lu, Y Liu, J Wang, TD Spector, R Saffery, JM Craig, JT Bell
Genome Medicine | BMC | Published : 2017
Abstract
Background: The association of in vitro fertilisation (IVF) and DNA methylation has been studied predominantly at regulatory regions of imprinted genes and at just thousands of the ~28 million CpG sites in the human genome. Methods: We investigated the links between IVF and DNA methylation patterns in whole cord blood cells (n = 98) and cord blood mononuclear cells (n = 82) from newborn twins using genome-wide methylated DNA immunoprecipitation coupled with deep sequencing. Results: At a false discovery rate (FDR) of 5%, we identified one significant whole blood DNA methylation change linked to conception via IVF, which was located ~3 kb upstream of TNP1, a gene previously linked to male inf..
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Awarded by Biotechnology and Biological Sciences Research Council
Funding Acknowledgements
PETS was supported by grants from the Australian National Health and Medical Research Council (grant numbers 437015 and 607358 to JC and RS), the Financial Markets Foundation for Children (grant number 032-2007) and by the Victorian Government's Operational Infrastructure Support Program. RS is supported by a NHMRC Senior Research Fellowship. JCF was funded by EU-FP7 project EpiTrain (316758). TDS is an NIHR Senior Investigator and a European Research Council Senior Research Investigator (ERC 250157). The study also received support from the TwinsUK resource, funded by the Wellcome Trust, with support from the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility and Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust in partnership with King's College London. The study was also supported by the Biotechnology and Biological Sciences Research Council (BBSRC) and the Economic and Social Research Council (ESRC) (grant number ES/N000404/1 to JTB).