Journal article

The elaborate route for UDP-arabinose delivery into the Golgi of plants

Carsten Rautengarten, Devon Birdseye, Sivakumar Pattathil, Heather E McFarlane, Susana Saez-Aguayo, Ariel Orellana, Staffan Persson, Michael G Hahn, Henrik V Scheller, Joshua L Heazlewood, Berit Ebert

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2017

Abstract

In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptides. Whereas the majority of Ara found in plant glycans occurs as a furanose ring (Araf), the activated precursor has a pyranose ring configuration (UDP-Arap). The biosynthesis of UDP-Arap mainly occurs via the epimerization of UDP-xylose (UDP-Xyl) in the Golgi lumen. Given that the predominant Ara form found in plants is Araf, UDP-Arap must exit the Golgi to be interconverted into UDP-Araf by UDP-Ara mutases that are located outside on the cytosolic surface of the Golgi. Subsequently, UDP-Araf must be transported back into the lumen. This step is vital because glyc..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by ARC


Awarded by US Department of Energy


Awarded by Lawrence Berkeley National Laboratory


Awarded by Fondecyt


Awarded by Fondo de Areas Prioritarias-Centro de Regulacion del Genoma


Awarded by Committee of Evaluation and Direction of the Scientific Cooperation-CONICYT


Awarded by Basal Program


Awarded by NSF


Awarded by NSF-Research Coordination Networks


Funding Acknowledgements

J.L.H., B.E., and S. Persson are supported by Australian Research Council (ARC) Future Fellowships (FT130101165, FT160100276, and FT160100218). H.E.M. is supported by an ARC Discovery Early Career Researcher Award (DE170100054). Microscopy was supported by an ARC Linkage Infrastructure, Equipment, and Facilities (LE150100011) and Biological Optical Microscopy Platform (The University of Melbourne). The work was supported by the US Department of Energy through contract DE-AC02-05CH11231 with Lawrence Berkeley National Laboratory and contract DE-AC05-00OR22725 with Oak Ridge National Laboratory. A.O. is supported by Fondecyt 1151335, Fondo de Areas Prioritarias-Centro de Regulacion del Genoma 15090007, Committee of Evaluation and Direction of the Scientific Cooperation-CONICYT C14B02, and Basal Program PB-16. S.S.-A. is supported by Fondecyt 11160787. The generation of the Complex Carbohydrate Research Center series of plant cell wall glycan-directed antibodies was supported by the NSF Plant Genome Program (DBI-0421683 and IOS-0923992). Substrates from CarboSource Services (Athens, GA) were supported by NSF-Research Coordination Networks Grant 0090281.