Characterisation by multilocus sequence and porA and flaA typing of Campylobacter jejuni isolated from samples of dog faeces collected in one city in New Zealand
V Mohan, MA Stevenson, JC Marshall, NP French
NEW ZEALAND VETERINARY JOURNAL | TAYLOR & FRANCIS LTD | Published : 2017
AIMS: To investigate the prevalence of Campylobacter spp. and C. jejuni in dog faecal material collected from dog walkways in the city of Palmerston North, New Zealand, and to characterise the C. jejuni isolates by multilocus sequence typing (MLST) and porA and flaA antigen gene typing. METHODS: A total of 355 fresh samples of dogs faeces were collected from bins provided for the disposal of dog faeces in 10 walkways in Palmerston North, New Zealand, between August 2008-July 2009. Presumptive Campylobacter colonies, cultured on modified charcoal cefoperazone deoxycholate plates, were screened for genus Campylobacter and C. jejuni by PCR. The C. jejuni isolates were subsequently characterised..View full abstract
The authors acknowledge the contributions of Julie Collins-Emerson and Anne Midwinter (mEpiLab), and Phil Carter (Institute of Environmental Science Research, New Zealand) for allele sequencing and assignment and all the technicians, particularly Sarah Moore, from mEpiLab, Institute of Veterinary, Animal and Biomedical Sciences, Massey University. Funding was also provided for V Mohan from a Massey University Doctoral Scholarship and a New Zealand International Doctoral Scholarship.