G M3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women

JM Wentworth; G Naselli; K Ngui; GK Smyth; R Liu; PE O'Brien; C Bruce; J Weir; M Cinel; PJ Meikle; LC Harrison

Journal article

G M3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women

JM Wentworth, G Naselli, K Ngui, GK Smyth, R Liu, PE O'Brien, C Bruce, J Weir, M Cinel, PJ Meikle, LC Harrison

International Journal of Obesity | NATURE PUBLISHING GROUP | Published : 2016

DOI: 10.1038/ijo.2015.223

Abstract

Aims:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.Methods:Subcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, lipid and gene profiling were performed.Results:In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in G M3 ganglioside and its synthesis enzyme ST3GAL..

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University of Melbourne Researchers

John Wentworth Author

Gordon Smyth Author

Peter Meikle Author

Leonard Harrison Author

Grants

Funding Acknowledgements

We are grateful to the women who participated in this study. The study was supported by a Diabetes Australia Research Trust (DART) Award, the Victorian State Government Operational Infrastructure Support and the Australian Government NHMRC IRIISS. JMW takes full responsibility for the work as a whole, including the study design, access to data and the decision to submit and publish the manuscript.