In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Stefanie Dudczig, Peter D Currie, Lucia Poggi, Patricia R Jusuf
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | JOURNAL OF VISUALIZED EXPERIMENTS | Published : 2017
The genetic and technical strengths have made the zebrafish vertebrate a key model organism in which the consequences of gene manipulations can be traced in vivo throughout the rapid developmental period. Multiple processes can be studied including cell proliferation, gene expression, cell migration and morphogenesis. Importantly, the generation of chimeras through transplantations can be easily performed, allowing mosaic labeling and tracking of individual cells under the influence of the host environment. For example, by combining functional gene manipulations of the host embryo (e.g., through morpholino microinjection) and live imaging, the effects of extrinsic, cell nonautonomous signals..View full abstract
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Awarded by ARC DECRA
Awarded by Deutsche Forschungsgemeinschaft (DFG)
This work was supported by an ARC DECRA to PRJ (DE120101311) and by a Deutsche Forschungsgemeinschaft (DFG) research grant to LP (PO 1440/1-1). The Australian Regenerative Medicine Institute is supported by funds from the State Government of Victoria and the Australian Federal Government. We acknowledge Dr Jeremy Ng Chi Kei, who conducted experiments described here as published in Kei et al., 2016. We are grateful for provisions of transgenic fish from Prof. Higashijima and thank Profs. Turner and Rupp for the provision of the pCS2+ plasmid and Dr. Wilkinson for generating H2B-RFP and H2A-GFP constructs. We thank FishCore facility staff (Monash University) for taking care of our animals.