Book Chapter

Culturing and Cryobanking Human Neural Stem Cells

Jeremy M Crook, Eva Tomaskovic-Crook

STEM CELL BANKING: CONCEPTS AND PROTOCOLS | Methods in Molecular Biology | HUMANA PRESS INC | Published : 2017


The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.