Book Chapter

Cryobanking Pluripotent Stem Cells

Jeremy M Crook, Eva Tomaskovic-Crook, Tenneille E Ludwig

STEM CELL BANKING: CONCEPTS AND PROTOCOLS | Methods in Molecular Biology | HUMANA PRESS INC | Published : 2017

Abstract

Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryo..

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