Journal article

Methylation profiling of paediatric pilocytic astrocytoma reveals variants specifically associated with tumour location and predictive of recurrence

Alexandra Sexton-Oates, Andrew Dodgshun, Volker Hovestadt, David TW Jones, David M Ashley, Michael Sullivan, Duncan MacGregor, Richard Saffery



Childhood pilocytic astrocytomas (PA) are low-grade tumours with an excellent prognosis. However, a minority, particularly those in surgically inaccessible locations, have poorer long-term outcome. At present, it is unclear whether anatomical location in isolation, or in combination with underlying biological variation, determines clinical behaviour. Here, we have tested the utility of DNA methylation profiling to inform tumour biology and to predict behaviour in paediatric PA. Genome-wide DNA methylation profiles were generated for 117 paediatric PAs. Using a combination of analyses, we identified DNA methylation variants specific to tumour location and predictive of behaviour. Receiver-ope..

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Funding Acknowledgements

We wish to thank Hazel Chambers-Smith for providing the immunohistochemistry service. This work was supported by The Cancer in Kids Auxiliary at The Royal Children's Hospital, Victoria, Australia; The Murdoch Childrens Research Institute, Victoria, Australia; The Victorian Government's Operational Infrastructure Support Program; an Australian National Health and Medical Research Council Senior Research Fellowship to RS; and an Australian Postgraduate Award to ASO.